Translational_Unit

Part:BBa_K5179010:Design

Designed by: Caden Sanko   Group: iGEM24_William-and-Mary   (2024-09-28)


Sid Operon Polycistron


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 798
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sid stop codon and start codon of delta overlap by one base pair. Sid open reading frame appears to contain a cryptic RBS for the downstream delta gene.


Source

Fragment from bacteriophage P4 essential region. DNA sourced from P4 cosmid (Addgene plasmid # 196335), which was a gift from Baojun Wang.

References